|
KMID : 0360319940260020231
|
|
Journal of Korean Cancer Research Association
1994 Volume.26 No. 2 p.231 ~ p.235
|
|
|
Development of GST-P+ Foci in 3 Week Old Rats Induced by Ditthylnitrosamine: Preliminary data for liver foci model
|
|
장자준
이윤실/이미숙/김태환
|
|
|
Abstract
|
|
|
A new medium-term in vivo hepatocarcinogenesis model was tried using GST-P+ hepatic foci. The treatment protocol was the i. p. injection of 50mg/kg body weight of diethylnitrosamine(DEN) once, twice or three times per week with or without 0.05%
phenobarbital(PB) in 3 week female Sprague-Dawley rats or male Fischer 344 rats. After 9 weeks, the rats were sacrificed and their livers were examined for immunohistochemically detectable GST-P+ foci. DEN treatment in 3 week old rats without PB
was
enough to induce GST-P+ foci. Protocol is very simple and convenient compared to other liver foci model using newborn rats or combined partial hepatectomy. And the yield of GST-P+ foci treated with DEN and PB was almost equal as other models.
Therefore,
this simple hepatic foci model may be useful for screening of carcinogenic or anticarcinogenic agents. For the validity of new model, more extensive studies will be necessary.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|